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human adult small intestine total rna  (TaKaRa)


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    TaKaRa human adult small intestine total rna
    Human Adult Small Intestine Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+adult+small+intestine+total+rna/Human+Small+Intestine+Total+RNA/pm40199084-80-16-22
    Average 94 stars, based on 141 article reviews
    human adult small intestine total rna - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Characterization and Structure of Alternatively Spliced Transcript Variant of Human Intestinal Alkaline Phosphatase (ALPI) Gene.
    Article Snippet: Total RNA from the cells was extracted by the thiocyanate-phenol-chloroform extraction method (CS104 RNAzolTM B, Tel-Test, Inc., Friendswood, TX, USA) (24). .. Human adult small intestine total RNA was purchased from Takara Bio USA, Inc. As a template for PCR, single-strand cDNA was prepared from 1 mg of total RNA using PrimeScriptTM II 1st strand cDNA synthesis Kit (Takara Bio, Shiga, Japan). ..

    cDNA Synthesis:

    Article Title: Characterization and Structure of Alternatively Spliced Transcript Variant of Human Intestinal Alkaline Phosphatase (ALPI) Gene.
    Article Snippet: Total RNA from the cells was extracted by the thiocyanate-phenol-chloroform extraction method (CS104 RNAzolTM B, Tel-Test, Inc., Friendswood, TX, USA) (24). .. Human adult small intestine total RNA was purchased from Takara Bio USA, Inc. As a template for PCR, single-strand cDNA was prepared from 1 mg of total RNA using PrimeScriptTM II 1st strand cDNA synthesis Kit (Takara Bio, Shiga, Japan). ..

    Expressing:

    Article Title: Human pluripotent stem cell-derived intestinal organoids for pharmacokinetic studies.
    Article Snippet: .. Measurements were normalized internally to β-ACTIN and GAPDH Expression levels, then externally against measurements from the Human Adult Small Intestine total RNA (Takara Bio, Japan) Lot No. 1901903A. ..



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    Fig. 1. Differentiation of human iPSCs into intestinal organoids (IOs). (A) A schematic drawing of the overall culture timeline of iPSCs to establish IOs, showing respective culture platforms, matrices, and key molecules at each stage of differentiation. (B) Bright-field images of iPSC-derived differentiated cells. (Upper) Bright- field images showing iPSCs in 3D cluster microwell culture (day 0, 3, 8) (Representative 2 wells are shown). (Lower) iPSC-derived IOs (iPSC-IOs) formed in Matrigel- based culture on differentiation days 15, 22, and 29. (Right table) Cell growth rates in passage 1 (P1) on day 22 (d22) and P2 on d29 are shown as fold-change in cell numbers after 7 days of culture versus the starting cell no. of 1.0 × 105 cells. Scale bars: 200 μm (upper), 500 μm (lower). (C) Relative gene expression level of marker genes analyzed by qPCR on day 0 (before differentiation), day 3 (endoderm), day 8 (mid-/hind-gut), day 15 (P0), day 22 (P1), and day 29 (P2) are shown. The qPCR data are normalized against the adult <t>small</t> <t>intestine</t> <t>(ASI</t> = 1). Data are expressed as the mean ± SD (standard deviation), N = 3. Significances are shown as *p < 0.05, or **p < 0.01, by Dunnett’s multiple comparisons tests compared with control ASI in each gene set.
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    Fig. 1. Differentiation of human iPSCs into intestinal organoids (IOs). (A) A schematic drawing of the overall culture timeline of iPSCs to establish IOs, showing respective culture platforms, matrices, and key molecules at each stage of differentiation. (B) Bright-field images of iPSC-derived differentiated cells. (Upper) Bright- field images showing iPSCs in 3D cluster microwell culture (day 0, 3, 8) (Representative 2 wells are shown). (Lower) iPSC-derived IOs (iPSC-IOs) formed in Matrigel- based culture on differentiation days 15, 22, and 29. (Right table) Cell growth rates in passage 1 (P1) on day 22 (d22) and P2 on d29 are shown as fold-change in cell numbers after 7 days of culture versus the starting cell no. of 1.0 × 105 cells. Scale bars: 200 μm (upper), 500 μm (lower). (C) Relative gene expression level of marker genes analyzed by qPCR on day 0 (before differentiation), day 3 (endoderm), day 8 (mid-/hind-gut), day 15 (P0), day 22 (P1), and day 29 (P2) are shown. The qPCR data are normalized against the adult <t>small</t> <t>intestine</t> <t>(ASI</t> = 1). Data are expressed as the mean ± SD (standard deviation), N = 3. Significances are shown as *p < 0.05, or **p < 0.01, by Dunnett’s multiple comparisons tests compared with control ASI in each gene set.
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    Fig. 1. Differentiation of human iPSCs into intestinal organoids (IOs). (A) A schematic drawing of the overall culture timeline of iPSCs to establish IOs, showing respective culture platforms, matrices, and key molecules at each stage of differentiation. (B) Bright-field images of iPSC-derived differentiated cells. (Upper) Bright- field images showing iPSCs in 3D cluster microwell culture (day 0, 3, 8) (Representative 2 wells are shown). (Lower) iPSC-derived IOs (iPSC-IOs) formed in Matrigel- based culture on differentiation days 15, 22, and 29. (Right table) Cell growth rates in passage 1 (P1) on day 22 (d22) and P2 on d29 are shown as fold-change in cell numbers after 7 days of culture versus the starting cell no. of 1.0 × 105 cells. Scale bars: 200 μm (upper), 500 μm (lower). (C) Relative gene expression level of marker genes analyzed by qPCR on day 0 (before differentiation), day 3 (endoderm), day 8 (mid-/hind-gut), day 15 (P0), day 22 (P1), and day 29 (P2) are shown. The qPCR data are normalized against the adult <t>small</t> <t>intestine</t> <t>(ASI</t> = 1). Data are expressed as the mean ± SD (standard deviation), N = 3. Significances are shown as *p < 0.05, or **p < 0.01, by Dunnett’s multiple comparisons tests compared with control ASI in each gene set.
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    Image Search Results


    Fig. 1. Differentiation of human iPSCs into intestinal organoids (IOs). (A) A schematic drawing of the overall culture timeline of iPSCs to establish IOs, showing respective culture platforms, matrices, and key molecules at each stage of differentiation. (B) Bright-field images of iPSC-derived differentiated cells. (Upper) Bright- field images showing iPSCs in 3D cluster microwell culture (day 0, 3, 8) (Representative 2 wells are shown). (Lower) iPSC-derived IOs (iPSC-IOs) formed in Matrigel- based culture on differentiation days 15, 22, and 29. (Right table) Cell growth rates in passage 1 (P1) on day 22 (d22) and P2 on d29 are shown as fold-change in cell numbers after 7 days of culture versus the starting cell no. of 1.0 × 105 cells. Scale bars: 200 μm (upper), 500 μm (lower). (C) Relative gene expression level of marker genes analyzed by qPCR on day 0 (before differentiation), day 3 (endoderm), day 8 (mid-/hind-gut), day 15 (P0), day 22 (P1), and day 29 (P2) are shown. The qPCR data are normalized against the adult small intestine (ASI = 1). Data are expressed as the mean ± SD (standard deviation), N = 3. Significances are shown as *p < 0.05, or **p < 0.01, by Dunnett’s multiple comparisons tests compared with control ASI in each gene set.

    Journal: European journal of cell biology

    Article Title: Human pluripotent stem cell-derived intestinal organoids for pharmacokinetic studies.

    doi: 10.1016/j.ejcb.2025.151489

    Figure Lengend Snippet: Fig. 1. Differentiation of human iPSCs into intestinal organoids (IOs). (A) A schematic drawing of the overall culture timeline of iPSCs to establish IOs, showing respective culture platforms, matrices, and key molecules at each stage of differentiation. (B) Bright-field images of iPSC-derived differentiated cells. (Upper) Bright- field images showing iPSCs in 3D cluster microwell culture (day 0, 3, 8) (Representative 2 wells are shown). (Lower) iPSC-derived IOs (iPSC-IOs) formed in Matrigel- based culture on differentiation days 15, 22, and 29. (Right table) Cell growth rates in passage 1 (P1) on day 22 (d22) and P2 on d29 are shown as fold-change in cell numbers after 7 days of culture versus the starting cell no. of 1.0 × 105 cells. Scale bars: 200 μm (upper), 500 μm (lower). (C) Relative gene expression level of marker genes analyzed by qPCR on day 0 (before differentiation), day 3 (endoderm), day 8 (mid-/hind-gut), day 15 (P0), day 22 (P1), and day 29 (P2) are shown. The qPCR data are normalized against the adult small intestine (ASI = 1). Data are expressed as the mean ± SD (standard deviation), N = 3. Significances are shown as *p < 0.05, or **p < 0.01, by Dunnett’s multiple comparisons tests compared with control ASI in each gene set.

    Article Snippet: Total RNA of the human adult small intestine (ASI) (Takara Bio, Cat. No. 636539, Lot.

    Techniques: Derivative Assay, Gene Expression, Marker, Standard Deviation, Control